Present study was undertaken to elucidate the effect of concentrate supplementation on the periparturient blood biochemical profiles of native ewes, two months before expected date of lambing in coastal Odisha. Forty (40) pregnant ewes were randomly distributed into two treatment groups (T1 and T2) based on their body weight, age and parity. First group (T1) was maintained on grazing only, while the second group (T2) was supplemented with a balanced concentrate mixture @ 200 g/sheep/day along with grazing. Blood samples were collected one month before and one month after parturition for analysis of serum glucose, total protein, albumin, globulin, A/G, cholesterol, triglyceride, urea and creatinine. Significant (P<0.05) increase was observed for serum glucose and globulin, both before and after parturition in (T2) (before: 56.65 ± 1.86 mg/dl and 5.08 ± 0.59 g/dl; after: 53.93 ± 1.62 mg/dl and 4.04 ± 0.32 g/dl) as compared to T1 (before: 51.23 ± 1.90 and 4.12 ± 0.45g/dl; after: 45.88 ± 1.57 mg/dl and 3.25 ± 0.45 g/dl). Non significant results were observed for rest of the biochemical parameters studied between the control and the treatment both before and after lambing. It was concluded that prepartum concentrate feeding @ 200 g /day/ewe is required to maintain the glucose and globulin level in ewes after compensating the energy needs of growing foetus and colostrum production in mammary gland.